做生物信息,遗传发育,分析数据的时候总是要narrow down分析范围,高通量数据尤其是基因表达,在庞大的confounder面前,缩小分析范围是必须的,否则你会一直在混沌中游荡. 看一篇文章:2018 - Identification of genes associated with Hirschsprung disease, based on whole-genome sequence analysis, and potential effects on enteric nervous s
Title: Single-Shot Capillary Zone Electrophoresis−Tandem Mass Spectrometry Produces over 4400 Phosphopeptide Identifications from a 220 ng Sample Journal: Journal of Proteome Research Published Date: June 10, 2019 DOI: 10.1021/acs.jproteome.9b00244 R
前言 本文主要演示GEO数据库的一些工具,使用的数据是2015年在Nature Communications上发表的文章Regulation of autophagy and the ubiquitin-proteasome system by the FoxO transcriptional network during muscle atrophy.[pubmed:25858807] 作者通过将FoxO1-3-4-floxed小鼠(FoxO1,3,4 f / f)与表达Cre重组酶的转基因系
NCBI(National Center for Biotechnology Information,美国国家生物技术信息中心)除了维护GenBank核酸序列数据库外,还提供数据分析和检索资源.NCBI资源包括Entrez.Entrez编程组件.MyNCBI.PubMed.PudMed Central.PubReader.Gene.the NCBI Taxonomy Browser.BLAST.Pimer-Blast.COBALT.RefSeq.UniGene.HomoloGene.ProtES
UCSC Genome Browser是由University of California Santa Cruz (UCSC) 创立和维护的,该站点包含有人类.小鼠和大鼠等多个物种的基因组草图和注释信息,并提供一系列的网页分析工具. 1.MySQL安装 本人使用的是Centos6.8系统,推荐使用yum安装. $ yum install -y mysql mysql-devel mysql-server 2.MySQLdb安装 MySQLdb是MySQL的一个线程兼容接口,提供了python数据
ENCODE: Encyclopedia of DNA Elements 目标:按不同组织,收集人类(还有小鼠.worm.fly)基因组里面的所有功能元件 The primary goal of the ENCODE project is to determine the role of the remaining component of the genome, much of which was traditionally regarded as "junk." 所包含的数据: 文