RegistryKey key = Registry.LocalMachine.OpenSubKey("SOFTWARE\\Microsoft\\Windows\\CurrentVersion\\Run", true);//打开注册表子项

key.SetValue(程序的名称, 程序的路径);

或

WshShell shell = new WshShell();

IWshShortcut shortcut = (IWshShortcut)shell.CreateShortcut(shortcutPath);

public void SetAutoRun(string fileName, bool isAutoRun)

{

    RegistryKey reg = null;

    try

    {

        if (!System.IO.File.Exists(fileName))

            throw new Exception("该文件不存在!");

        String name = fileName.Substring(fileName.LastIndexOf(@"\") + );

        reg = Registry.LocalMachine.OpenSubKey(@"SOFTWARE\Microsoft\Windows\CurrentVersion\Run", true);

        if (reg == null)

            reg = Registry.LocalMachine.CreateSubKey(@"SOFTWARE\Microsoft\Windows\CurrentVersion\Run");

        if (isAutoRun)

            reg.SetValue(name, fileName);

        else

            reg.SetValue(name, false);

        lbl_autorunerr.Visible = false;

    }

    catch

    {

        lbl_autorunerr.Visible = true;

        //throw new Exception(ex.ToString());

    }

    finally

    {

        if (reg != null)

            reg.Close();

    }

}