fastq,sam文件一些小结（持续补充。。。）
2024-10-11 16:03:39
ST-E00211::H5L3NCCXY:::: chr14 141M = - ACTTCACCTCCTGGAGTCCTGGACTTCCCCACATCTCCCCTGCCCCTCCCACGTTTCCATAGTCCAAGGGCCAGAGTAAATGAAAATACAGCAGCCGCCCAAGCAATGGGGCCCATGCTGGGGCTTCAGTCATCCCCATGT JFJFJFJJJJJJJJJJJJJJJFAJJJJJJJJJFFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFFFAA AS:i:- XN:i: XM:i: XO:i: XG:i: NM:i: MD:Z:0T0T132A0G3C1 YS:i:- YT:Z:CP
ST-E00211::H5L3NCCXY:::: chr14 141M = - ACTTCACCTCCTGGAGTCCTGGACTTCCCCACATCTCCCCTGCCCCTCCCACGTTTCCATAGTCCAAGGGCCAGAGTAAATGAAAATACAGCAGCCGCCCAAGCAATGGGGCCCATGCTGGGGCTTCAGTCATCCCCATGT A<FFFFFFFJJFJFJJ<<JJJJJJJJJJJJFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFJJJJJJFJJJ<JFJJJJJJJJAJJFJJJJJJJJJJJJJJJJJFJJJJFJJJJJJJJJJAJJJJJJJJJJJJJJJJ AS:i:- XN:i: XM:i: XO:i: XG:i: NM:i: MD:Z:0T0T132A0G3C1 YS:i:- YT:Z:CP
这是双端测序的sam文件随意挑出的一条测序片段。（sam文件会把同一片段的两条read都保留，从第二列可以知道是read1还是read2），可以发现这条片段已经被测通（因为染色体坐标一致），基因序列整体是相同的，说明read1和read2都是按5‘-->3’的顺序排列的
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